Monitoring the metabolomic profile of Arabidopsis seedlings treated with trans-chalcone
DOI:
https://doi.org/10.19084/rca.34863Abstract
The flavonoid trans-chalcone (chalcone, 1,3-diphenyl-2-propen-1-one) is a strong candidate for development of new strategies for weed control (bioherbicides). Previous studies have demonstrated its high phytotoxic capacity on Arabidopsis thaliana seedlings, inducing programmed cell death in roots after 14 days of growth in agar media supplemented with chalcone 35 µM. To determine the molecular mechanism of action of this phytotoxin, a metabolome monitorization was conducted on Arabidopsis seedlings treated with chalcone (0 and 35 µM) during 3, 10 and 20 hours. Samples were extracted from roots and shoots separately, derivatized and injected in a gas chromatography/mass spectrometry (GC/MS) equipment for untargeted metabolomics. Resulted data were analysed by software MS-DIAL for identification of compounds using public Mass Spectral of Electron Ionization Spectra and the Retention Index (RI) libraries. The annotated metabolites data were statistically analysed using Metaboanalyst 5.0 for PCA, two-way ANOVA, time-series multivariate analysis (MEBA), and for metabolic pathway analysis. Results indicated that chalcone affected Arabidopsis metabolome after only 3 hours of treatment. Most impacted routes were related to amino acid and carbon metabolism and were very similar in roots and shoots. However, a detailed approach revealed different mode of action of chalcone on roots and shoots.