Optimization of the in vitro plants production of Ipomoea batatas cultivars
DOI:
https://doi.org/10.19084/rca.16948Abstract
Due to the importance of sweet potato culture, this study aimed to optimize the production of the in vitro plants of different cultivars. Nodal segments of cultivars ‘Abóbora’, ‘Catarina’, ‘Biaze’, ‘Da Costa’, ‘Americana’ e ‘Morada Inta’ were cultured on Murashige and Skoog medium and they were evaluated for eight weeks in an in vitroculture. The cv. ‘Da Costa’ was also grown on a medium free of plant growth regulators, supplemented with benzylaminopurine (BA) and/or gibberellic acid (GA3) or in photoautotrophic culture system. It was found that the propagation rate is variable, depending on the cultivar and according to time of cultivation. The interval among subcultures was dependent on the rate of plant senescence. In cv. ‘Da Costa’, a higher production of plants was obtained on medium containing BAP or on sucrose-free medium and in bottles sealed with cotton. Plants originating from all cultivars showed a high survival rate during the acclimatization.