EXTRATO DAS SEMENTES DE MORINGA OLEIFERA APLICADOS EM EMULSÕES COSMÉTICAS COMO CONSERVANTE
DOI:
https://doi.org/10.25746/ruiips.v4.i4.14450Keywords:
Pseudomonas aeruginosa. microbial agent.Abstract
RESUMO
O trabalho investiga o efeito do extrato das sementes de moringa sobre o crescimento de Pseudomonas aeruginosa na produção de emulsão cosmética e a estabilidade do produto, sendo obtido por extração mecânica com prensagem hidráulica descontínua. Emulsão A/O não iônica foi produzida em bancada. As amostras testadas foram amostra A: metiparabeno, propilparabeno e imidazolidinil uréia, pH 5,63 a 21,6 0C e amostras B, C, D, E e F em concentrações decrescentes do extrato da semente Moringa, pH 5,4 a 20 0C. Colônias de Pseudomonas aeruginosa foram diluídas em salina 0,9% estéril para 108 UFC/mL segundo a escala 0,5 de Mac Farland (BAUER, 1966). Em tubos contendo 1 mL de caldo BHI, preparado em concentração simples, foram realizadas diluições 1:10 adicionando-se em 1 mL do caldo 0,1 mL da suspensão bacteriana e 0,1 mL das respectivas emulsões (A,B,C,D,E,F). Após 24 horas, foi estriado em placas em meio Ágar Cetrimide para realizar testes de confirmação de coloração de Gram. A contagem da carga microbiana foi realizada a 28 dias de inoculação e os resultados foram comparados com limites estabelecidos, para avaliar a eficácia do sistema conservante. Os resultados obtidos nos experimentos indicam consideravelmente a inibição do crescimento da Pseudomonas aeruginosas. As amostras B, C, D, E, e F não apresentaram aspecto modificado e os parâmetros de cor e odor permaneceram inalterados, atuando como preservante da fórmula cosmética.
ABSTRACT
This work investigates the effect of the extract of Moringa seeds on growth of Pseudomonas aeruginosa in the production of cosmetic emulsion and the stability of the product being obtained by mechanical extraction batch hydraulic press. W / O emulsion was produced nonionic bench. The samples tested were sample A: metiparabeno, propyl paraben and imidazolidinyl urea, pH 5.63 to 21.6 0C and samples B, C, D, E and F in decreasing concentrations of Moringa seed extract, pH 5.4 to 20 0C. Colonies of Pseudomonas aeruginosa were diluted in sterile 0.9% saline to 108 CFU / ml according to Mac Farland 0.5 scale (Bauer, 1966). In tubes containing 1 ml of BHI broth, prepared in simple concentration dilutions were performed by adding 1:10 in 1 ml of the broth 0.1 ml of the bacterial suspension and 0.1 ml of the respective emulsions (A, B, C, D, E, F). After 24 hours, the striatum was plated on cetrimide agar medium to perform Gram stain confirmation tests. The count of microbial load was determined within 28 days of inoculation and the results were compared with established limits, to assess the effectiveness of the preservative system. The results of the experiments indicate significantly inhibiting the growth of Pseudomonas aeruginosa. Samples B, C, D, E, and F had not changed appearance and color parameters and odor remained unchanged, acting as a preservative in cosmetic formula.
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